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Monday to Friday: 7AM - 7PM
Weekend: 10AM - 5PM
COURSE MATERIALS
Into the Blue
for transdisciplinary learning
Materials: Labwork
DNA / RNA degradation
General precautions when handling RNA. A text with illustration explaining: a) general concepts and function of RNA, DNA, RNAase; degradation of RNA; b) the major sources of RNase contamination in a typical laboratory; c) precautions to avoid RNase contamination.
Cutting DNA
This is a basic protocol to cut DNA into smaller fragments. The cut is nonspecific, being an excellent example of contrast with the specificity of CRISPR/cas9.
Electrophoresis
This is a protocol to do an agarose gel electrophoresis, a standard lab procedure used to separate macromolecules based on size. The technique applies a negative charge so proteins move towards a positive charge. Electrophoresis is used extensively in DNA, RNA, and protein analysis.
CRISPR-Cas9 editing of Streptomyces genome: Introduction
Presentation by Marta Vaz Mendes, microbiology researcher, explaining the main steps of editing Streptomyces with CRISPR-Cas9.
CRISPR – Cas9 editing of Streptomyces genome: Protocols
Protocols for in vivo triggering of CAS9 system to switch off blue pigment production in Streptomyces coelicolor.
In Living Color: Bacterial Pigments as an Untapped Resource in the Classroom and Beyond
Charkoudian, L. K., Fitzgerald, J. T., Khosla, C., & Champlin, A. (2010). In Living Color: Bacterial Pigments as an Untapped Resource in the Classroom and Beyond. PLOS Biology, 8(10), e1000510.
In silico lab work: CRISPR cas9 ge27ne editing, Fábio Ferreira (28’47’’)
Fábio Júnio Ferreira, i3S researcher, introduces how to design a sgRNA, particularly to edit Streptomycetes and zebrafish.
Guide for the synthesis of single-guide RNA (sgRNA)
Document with information, tools, and parameters to design sgRNA.
